Trivia: There is a scene when the lemurs are showing a book called "To Serve Lemur". This is similar to one of the classic episodes of The Twilight Zone, "To Serve Man" when the "aliens" allegedly come in peace but actually want to eat the people of earth.
Trivia: In the scene where Marty the Zebra is building his shelter, Alex gets jealous and looks to a basketball with a handprint face with grass for hair and says, "Shut up, Spalding". This is a reference to the film Cast Away, in which Tom Hanks' character, Chuck Noland, starts talking to a Wilson volleyball and gives it the name "Wilson." The name "Spalding" also references the dentist that Tom Hanks mentions in Cast Away to Wilson.
Trivia: When Alex (voiced by Ben Stiller) is taking pictures for the crowd in the beginning he strikes the famous "blue steel" pose from another one of Ben Stiller's movies, "Zoolander".
Trivia: The movie references the novel "The Lord of the Flies" several times: They land on an island and quickly split into two groups. One wants to signal for help, and the other wants to stay on the island. A line is drawn between the two sides. The dead parachuter in the trees is the directly-channeled "beastie" in the book. Maurice assumes the floor in the meeting by blowing the conch shell.
Trivia: In the German and Dutch translation, the chimps go to hear (and throw poo at) Hillary Clinton.
Trivia: When Phil the monkey signs the words "ship to wildlife preserve Kenya," he starts with "tell the stupid bird it says".
Trivia: Julien was only supposed to have two lines. But after Sacha Baron Cohen was cast in the role, he ad-libbed so much of his dialogue that he became a fundamental part of the entire series.
